Photochemical control of bacterial signal processing using a light-activated erythromycin.
نویسندگان
چکیده
Bacterial cells control resistance to the macrolide antibiotic erythromycin using the MphR(A) repressor protein. Erythromycin binds to MphR(A), causing release of the PmphR promoter, activating expression of the 2'-phosphotransferase Mph(A). We engineered the MphR(A)/promoter system to, in conjunction with a light-activatable derivative of erythromycin, enable photochemical activation of gene expression in E. coli. We applied this photochemical gene switch to the construction of a light-triggered logic gate, a light-controlled band-pass filter, as well as spatial and temporal control of gene expression.
منابع مشابه
Using Temperature of IR Sources for Assessing Photochemical and Aphakic Retinal Hazard
Introduction Blue light is a part of the spectrum with the highest energy content, which can reach the retina. The damage that it can cause to the retina is called photochemical or blue-light retinal injury. For the retinal injury assessment of the photochemical and aphakic retinal hazards in the wavelength range of 300-700 nm, use of effective spectral radiance limits (W.m-2.sr-1) seems to be ...
متن کاملPerformance of the Wavelet Transform-Neural Network Based Receiver for DPIM in Diffuse Indoor Optical Wireless Links in Presence of Artificial Light Interference
Artificial neural network (ANN) has application in communication engineering in diverse areas such as channel equalization, channel modeling, error control code because of its capability of nonlinear processing, adaptability, and parallel processing. On the other hand, wavelet transform (WT) with both the time and the frequency resolution provides the exact representation of signal in both doma...
متن کاملProtection of Archival Documents from Photochemical Eects
Purpose: The purpose of this paper is to highlight the destructive effects of light on archival documents/paper materials. The research aims to explain the mechanism of photochemical degradation and the damaging effect of light on paper. It also tells us about the measures to be adopted to control the deteriorating effects of light on paper step by step. Design/Methodology/Approach: The res...
متن کاملاثر شدت تابش بر تحمل به سرمای سویا [Glycine max (L.) Merr.] با استفاده از روش فلورسانس کلروفیل
In order to evaluate the effects of both cold and light stresses on chlorophyll fluorescence and feasibility of using chlorophyll florescence technique to evaluate effect of light intensity on cold tolerance in soybean, an experiment was done in a factorial arrangement based on a completely randomized design with three replicates. Two soybean cultivars consisted of 032 and BP grown under greenh...
متن کاملDamage detection and structural health monitoring of ST-37 plate using smart materials and signal processing by artificial neural networks
Structural health monitoring (SHM) systems operate online and test different materials using ultrasonic guided waves and piezoelectric smart materials. These systems are permanently installed on the structures and display information on the monitor screen. The user informs the engineers of the existing damage after observing signal loss which appears after damage is caused. In this paper health...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Molecular bioSystems
دوره 7 9 شماره
صفحات -
تاریخ انتشار 2011